GSM3791753: NSD2 High 1 H3K27ac-ChIPseq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
KMS-11
Primary Tissue
Blood
Tissue Diagnosis
Multiple Myeloma
Attributes by original data submitter
Sample
source_name
KMS11
parental cell line
KMS11
cell type
multiple myeloma cell line
genotype/variation
parental
kit
Nextera
chip antibody
H3K27ac (Abcam ab4729)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA was extracted using the RNeasy Mini Kit from Qiagen (Cat. No. 74104) in accordance with the prescribed protocol provided with the kit. For ATAC-seq,ChIP-seq and HiChIP, sequencing libraries were constructed using the Illumina Nextera DNA Sample prep kit. RNA libraries were prepared for sequencing using standard Illumina protocols. Hi-C libraries were prepared as per the Arima kit guidelines. RNAseq, ChIP-seq, ATAC-seq, Hi-C, HiChIP, 4C