Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Blood

Cell type

KMS-11

Primary Tissue

Blood

Tissue Diagnosis

Multiple Myeloma

Sample

source_name

KMS11

parental cell line

KMS11

cell type

multiple myeloma cell line

genotype/variation

parental

kit

Nextera

chip antibody

CTCF (Millipore 07-729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Total RNA was extracted using the RNeasy Mini Kit from Qiagen (Cat. No. 74104) in accordance with the prescribed protocol provided with the kit. For ATAC-seq,ChIP-seq and HiChIP, sequencing libraries were constructed using the Illumina Nextera DNA Sample prep kit. RNA libraries were prepared for sequencing using standard Illumina protocols. Hi-C libraries were prepared as per the Arima kit guidelines. RNAseq, ChIP-seq, ATAC-seq, Hi-C, HiChIP, 4C

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

25529872

Reads aligned (%)

97.5

Duplicates removed (%)

53.3

Number of peaks

32790 (qval < 1E-05)

hg19

Number of total reads

25529872

Reads aligned (%)

96.8

Duplicates removed (%)

53.7

Number of peaks

32411 (qval < 1E-05)