Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCF

Cell type

Cell type Class
Blood
Cell type
KMS-11
Primary Tissue
Blood
Tissue Diagnosis
Multiple Myeloma

Attributes by original data submitter

Sample

source_name
KMS11
parental cell line
KMS11
cell type
multiple myeloma cell line
genotype/variation
parental
kit
Nextera
chip antibody
CTCF (Millipore 07-729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA was extracted using the RNeasy Mini Kit from Qiagen (Cat. No. 74104) in accordance with the prescribed protocol provided with the kit. For ATAC-seq,ChIP-seq and HiChIP, sequencing libraries were constructed using the Illumina Nextera DNA Sample prep kit. RNA libraries were prepared for sequencing using standard Illumina protocols. Hi-C libraries were prepared as per the Arima kit guidelines. RNAseq, ChIP-seq, ATAC-seq, Hi-C, HiChIP, 4C

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
25529872
Reads aligned (%)
89.6
Duplicates removed (%)
53.7
Number of peaks
32411 (qval < 1E-05)

hg38

Number of total reads
25529872
Reads aligned (%)
91.1
Duplicates removed (%)
53.3
Number of peaks
32790 (qval < 1E-05)

Base call quality data from DBCLS SRA