Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

ChIP using anti-Flag M2 antibody (Sigma) in U2OS Flag-Z2 cells

cell line

U2OS Flag-Z2 cells

chip antibody

anti-Flag M2 antibody (Sigma)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were isolated and chromatin was sonicated and precleared. The H2A.Z histone-DNA complexes were isolated using anti-Flag M2 antibody (Sigma). Spike-in chromatin and Spike-in antibody (Active motif) were added in the ChIP experiment. After purification, 10 ng of immunoprecipitated DNA was sent to EMBL Genecore (Heidelberg, Germany) for library construction and sequencing. Library construction (NEBNext® Ultra™ II DNA Library Prep Kit for Illumina) was performed by EMBL GeneCore. The 4 samples were barcoded and sequenced in one lane by NextSeq 500 Illumina device.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

135043090

Reads aligned (%)

97.7

Duplicates removed (%)

26.4

Number of peaks

156507 (qval < 1E-05)

hg19

Number of total reads

135043090

Reads aligned (%)

97.3

Duplicates removed (%)

27.0

Number of peaks

155509 (qval < 1E-05)