Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Hematopoietic progenitors
NA
NA

Attributes by original data submitter

Sample

source_name
Hoxa9/Meis1-transformed HSCs_input
strain background
C57B6
tissue
bone marrow
cell type
hematopoietic progenitor cells
transduced with
MIGR1-HA-Hoxa9 and MIGR1-Flag-Meis1
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated cells and histone-DNA complexes were isolated with antibody. Immunoprecipitated chromatin was RNase treated and column purified. Libraries were generated using Illumina ChIP-seq Library Preparation Kit

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
54768760
Reads aligned (%)
75.5
Duplicates removed (%)
15.7
Number of peaks
571 (qval < 1E-05)

mm9

Number of total reads
54768760
Reads aligned (%)
75.2
Duplicates removed (%)
15.7
Number of peaks
630 (qval < 1E-05)

Base call quality data from DBCLS SRA