Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Blood

Cell type

Hematopoietic progenitors

NA

NA

Sample

source_name

Hoxa9/Meis1-transformed HSCs_ChIP-seq

strain background

C57B6

tissue

bone marrow

cell type

hematopoietic progenitor cells

transduced with

MIGR1-HA-Hoxa9 and MIGR1-Flag-Meis1

chip antibody

anti-HA

chip antibody vendor

Abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated cells and histone-DNA complexes were isolated with antibody. Immunoprecipitated chromatin was RNase treated and column purified. Libraries were generated using Illumina ChIP-seq Library Preparation Kit

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

43378206

Reads aligned (%)

78.3

Duplicates removed (%)

25.0

Number of peaks

892 (qval < 1E-05)

mm9

Number of total reads

43378206

Reads aligned (%)

78.0

Duplicates removed (%)

25.0

Number of peaks

945 (qval < 1E-05)