GSM3780672: BPLER DMSO Input ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
BLaER2
NA
NA
Attributes by original data submitter
Sample
source_name
stem-like breast cancer cells
cell line
BPLER2
cell type
a stem-like breast cancer (BrCa) cell model
transforming gene
TERT; SV40-LT/st; HRASV12
treatment
DMSO
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates from formaldehyde cross-linked cells were clarified from sonicated nuclei and histone-DNA complexes were immunoprecipitated with antibody. Cross-links were reversed and DNA extracted for library construction. Libraries for paired-end sequencing and alignment were prepared according to NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® according to manufacturer instructions (New England BioLabs).