Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Elba2

Cell type

Cell type Class
Embryo
Cell type
Early embryos
NA
NA

Attributes by original data submitter

Sample

source_name
Drosophila early embryo
genotype/variation
insv mutant
tissue
early embyro
age
2-4 hours
molecule subtype
ChIPed genomic DNA
antibody
Elba2 antibody in ChIP

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Standard protocols were used to make chromatin extract for ChIP. Total RNAs were extracted with Trizol reagent for RNA-seq. Standard PRO-seq protocol was used to make PRO-seq libraries ChIP-seq libraries were made using NEB DNA library prep kit. ChIP-nexus library was made as previouly described (He, et al ,2015). RNA-seq libraries were made by using illumina True-seq total RNA library kit LT. PRO-seq libraries were made as previously described (Kwak, et al, 2013).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
11757221
Reads aligned (%)
26.9
Duplicates removed (%)
62.8
Number of peaks
1275 (qval < 1E-05)

dm6

Number of total reads
11757221
Reads aligned (%)
25.5
Duplicates removed (%)
64.5
Number of peaks
1569 (qval < 1E-05)

Base call quality data from DBCLS SRA