Curated Sample Data


Genome
dm3
Antigen Class
TFs and others
Antigen
Elba2
Cell type Class
Embryo
Cell type
Early embryos

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
Drosophila early embryo
genotype/variation
elba3 mutant
tissue
early embyro
age
2-4 hours
molecule subtype
ChIPed genomic DNA
antibody
Elba2 antibody in ChIP

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Standard protocols were used to make chromatin extract for ChIP. Total RNAs were extracted with Trizol reagent for RNA-seq. Standard PRO-seq protocol was used to make PRO-seq libraries ChIP-seq libraries were made using NEB DNA library prep kit. ChIP-nexus library was made as previouly described (He, et al ,2015). RNA-seq libraries were made by using illumina True-seq total RNA library kit LT. PRO-seq libraries were made as previously described (Kwak, et al, 2013).

Platform Information


instrument_model
Illumina HiSeq 2500

External Database Query

Logs in read processing pipeline


Number of total reads
13130221
Reads aligned (%)
74.7
Duplicates removed (%)
56.6
Number of peaks
1471 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA