Antigen

Antigen Class

TFs and others

Antigen

AML1-ETO

Cell type

Cell type Class

Blood

Cell type

Kasumi-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Kasumi1-shTAF1-AE

cell line

Kasumi-1

cell type

Acute Myeloid Leukemia

genotype/variation

shTAF1-AE

chip antibody

AML1-ETO polyclonal antibody (Diagenode, C15310197)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

SimpleChIP Enzymatic Chromatin IP kit (Cell Siganling Technology) was used for chromatin immunoprecipitation per manufacturers instructions. Libraries were prepared using E7645S NEBNext(r) Ultra(tm) II DNA Library Prep Kit for Illumina® accoring to manufacturers instructions.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

15413887

Reads aligned (%)

96.5

Duplicates removed (%)

8.6

Number of peaks

6336 (qval < 1E-05)

hg19

Number of total reads

15413887

Reads aligned (%)

95.5

Duplicates removed (%)

10.0

Number of peaks

6270 (qval < 1E-05)