GSM3756721: LN229/EGFRvIII+erlotinib input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Neural
Cell type
LN-229
Primary Tissue
Brain
Tissue Diagnosis
Glioma
Attributes by original data submitter
Sample
source_name
brain
genotype/variation
stable expression of EGFRvIII
cell line
LN229
cell type
glioblastoma
tissue
brain
antibody
input
treatment
10 uM erlotinib
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
LN229 cells were cross-linked with formaldehyde to a final concentration of 1%. ChIP samples were prepared as described previously [(Buschbeck et al. The histone variant macroH2A is an epigenetic regulator of key developmental genes. Nat Struct Mol Biol 16, 1074-1079 (2009)].Immunoprecipitations were performed using 1μg anti- macroH2A1 antibody (07-219, Millipore) or the relevant non-specific IgG (Santa Cruz). ChIP-seq libraries generation were performed according to standard protocols using BioScientific's DNA Sample Kit.