Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
POLR2C

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293
cell line
HEK293-tagged RPB1
clone
#19
treatment
Dox and auxin treated
chip antibody
RPB3 (rabbit polyclonal antibody from Bethyl laboratories (RPB3 Antibody, A303-771A))

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin lysates were prepared by sonication and protein-DNA complexes were immunoprecipitated. ChIPseq libraries were prepared from 5-10 ng ChIP DNA following the instructions of Illumina TruSeq ChIP Library Preparation Kit (IP-202-1012).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
39834464
Reads aligned (%)
92.7
Duplicates removed (%)
5.2
Number of peaks
1080 (qval < 1E-05)

hg38

Number of total reads
39834464
Reads aligned (%)
94.8
Duplicates removed (%)
3.9
Number of peaks
1238 (qval < 1E-05)

Base call quality data from DBCLS SRA