Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Embryo
Cell type
Mitotic cycle 14

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
Drosophila melanogaster embryo
tissue
embryo
chip-antibody
NA
embryonic stage
early nuclear cycle 14
genotype
(UAS-OptoSOS/gal4);(CicsfGFP/gal4)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryos were sonicated 7 times, 20 seconds each, at 20% output. Cic-DNA complexes were isolated using magnetic protein G dynabead and GFP antibody. DNA was uncrosslinked from protein and purified. Purified ChIP and input DNA were prepared for Illumina sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S). The primers used for multiplexing the library came from the NEBNext Multiplex Oligos for Illumina Kit (E7335S).

Platform Information


instrument_model
Illumina HiSeq 2500

External Database Query

Logs in read processing pipeline


Number of total reads
20826584
Reads aligned (%)
89.5
Duplicates removed (%)
67.4
Number of peaks
4523 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA