Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
Mitotic cycle 14
NA
NA

Attributes by original data submitter

Sample

source_name
Drosophila melanogaster embryo
tissue
embryo
chip-antibody
NA
embryonic stage
early nuclear cycle 14
genotype
(UAS-OptoSOS/gal4);(CicsfGFP/gal4)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryos were sonicated 7 times, 20 seconds each, at 20% output. Cic-DNA complexes were isolated using magnetic protein G dynabead and GFP antibody. DNA was uncrosslinked from protein and purified. Purified ChIP and input DNA were prepared for Illumina sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S). The primers used for multiplexing the library came from the NEBNext Multiplex Oligos for Illumina Kit (E7335S).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm6

Number of total reads
17109992
Reads aligned (%)
95.8
Duplicates removed (%)
33.6
Number of peaks
2158 (qval < 1E-05)

dm3

Number of total reads
17109992
Reads aligned (%)
96.3
Duplicates removed (%)
29.4
Number of peaks
2404 (qval < 1E-05)

Base call quality data from DBCLS SRA