GSM3743625: A2B5+ iOPC(H) input Rep3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA
Attributes by original data submitter
Sample
source_name
Foreskin fibroblasts
tissue
Foreskin fibroblasts
cell line
A2B5+ iOPCs(H)
chip antibody
--
chip antibody vendor
--
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
H9-hESCs and A2B5+ iOPCs were cross-linked with 1% (wt/vol) formaldehyde fixation buffer, resuspended and lysed using an EZ-ChIP kit (Upstate, Millipore). To shear the cross-linked DNA, the lysates were subjected to 30 cycles of 30 seconds of sonication followed by 30 seconds of rest on ice with a Bioruptor sonicator (Diagenode, UCD-200) and incubated with protein G agarose beads and 3 μg of an anti-OCT4 antibody overnight at 4°C. After chromatin immunoprecipitation, the rest of the experiments were performed according to the manufacturer's protocols The construction of library was performed using NEBNext®UltraTM DNA Library Prep Kit for Illumina (New England Biolabs, UK) according to the manufacturer's instructions.