Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA

Attributes by original data submitter

Sample

source_name
Foreskin fibroblasts
tissue
Foreskin fibroblasts
cell line
A2B5+ iOPCs(H)
chip antibody
--
chip antibody vendor
--

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
H9-hESCs and A2B5+ iOPCs were cross-linked with 1% (wt/vol) formaldehyde fixation buffer, resuspended and lysed using an EZ-ChIP kit (Upstate, Millipore). To shear the cross-linked DNA, the lysates were subjected to 30 cycles of 30 seconds of sonication followed by 30 seconds of rest on ice with a Bioruptor sonicator (Diagenode, UCD-200) and incubated with protein G agarose beads and 3 μg of an anti-OCT4 antibody overnight at 4°C. After chromatin immunoprecipitation, the rest of the experiments were performed according to the manufacturer's protocols The construction of library was performed using NEBNext®UltraTM DNA Library Prep Kit for Illumina (New England Biolabs, UK) according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
29442368
Reads aligned (%)
50.8
Duplicates removed (%)
88.4
Number of peaks
131 (qval < 1E-05)

hg19

Number of total reads
29442368
Reads aligned (%)
50.2
Duplicates removed (%)
89.5
Number of peaks
108 (qval < 1E-05)

Base call quality data from DBCLS SRA