GSM3741662: 3668-SH-3 FOXO1 input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Blood
Cell type
OCI-LY1
NA
NA
Attributes by original data submitter
Sample
source_name
OCI-LY1 cells
growth media
IMDM +10%FBS
treatment
untreated
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
cells were cross-linked with 1% formaldehyde, quenched with glycine, washed 2x with PBS. Cells were lysed and nuclei harvested and resuspended in ChIP lysis buffer (25 mM tris, ph 8.0; 150 mM NaCl; 2mM EDTA, 1% Triton x-100; 0.25% SDS; protease inhibitors) prior to sonication on Biorupter (Diagenode). Samples were diluted two times in buffer without SDS and IP carried out with 5 μg antibody for 2 hours and pulled down with 15 μl 1:1 Prot A:Prot G Dynabeads first steps of library construction were carried out on beads including- polishing, A-tailing, P7 adapter ligation, Phi29 nick repair, PNK, lambda exonuclease treatnent, and RecJf nuclease treatment prior to elution and cross-link reversal. Library construction was completed by P7 primer extension and P5 adaptor ligation followed by AMPure purification and library amplification