GSM3741723: anti-SOX17 TCam-2 #3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SOX17
Cell type
Cell type Class
Gonad
Cell type
Tcam-2
NA
NA
Attributes by original data submitter
Sample
source_name
Human testicular germ cell tumour cell line
cell line
TCam-2
cell type
Seminoma-like cells
chip antibody
Anti-SOX17
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For chromatin preparation of cell lines, 1 x 10e7 cells / immunoprecipitation were fixed for 30 minutes (min) at room temperature (RT) using Diagenode Crosslink Gold (Diagenode, Liège, Belgium). After two washing steps with PBS (Thermo Fisher Scientific) cells were additionally fixed for 10 min in 1% formaldehyde (Applichem, Darmstadt, Germany) and then processed according to the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, Leiden, Netherlands). ChIP-seq libraries were generated using the MicroPlex Library Preparation Kit v2 (Diagenode).