GSM3741721: anti-SOX17 TCam-2 #2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
Human testicular germ cell tumour cell line
Sequenced DNA Library
For chromatin preparation of cell lines, 1 x 10e7 cells / immunoprecipitation were fixed for 30 minutes (min) at room temperature (RT) using Diagenode Crosslink Gold (Diagenode, Liège, Belgium). After two washing steps with PBS (Thermo Fisher Scientific) cells were additionally fixed for 10 min in 1% formaldehyde (Applichem, Darmstadt, Germany) and then processed according to the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, Leiden, Netherlands). ChIP-seq libraries were generated using the MicroPlex Library Preparation Kit v2 (Diagenode).