GSM3741716: 2% Input 2102EP #3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Gonad
Cell type
N2102Ep
NA
NA
Attributes by original data submitter
Sample
source_name
Human testicular germ cell tumour cell line
cell line
2102EP
cell type
Embryonal carcinoma-like cells
chip antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For chromatin preparation of cell lines, 1 x 10e7 cells / immunoprecipitation were fixed for 30 minutes (min) at room temperature (RT) using Diagenode Crosslink Gold (Diagenode, Liège, Belgium). After two washing steps with PBS (Thermo Fisher Scientific) cells were additionally fixed for 10 min in 1% formaldehyde (Applichem, Darmstadt, Germany) and then processed according to the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, Leiden, Netherlands). ChIP-seq libraries were generated using the MicroPlex Library Preparation Kit v2 (Diagenode).