Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
ATCC
cell type
K562
chip antibody
none (input)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are crosslinked 10' in 1%formaldehide, lysed and sonicated. The indicated mark is immunoprecipitated overnaight, and enriched by magnetical isolation. IP fraction is eluted and decrosslinked. Ovation Ultralow v2 (Nugen)

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
30523031
Reads aligned (%)
98.8
Duplicates removed (%)
4.5
Number of peaks
841 (qval < 1E-05)

hg19

Number of total reads
30523031
Reads aligned (%)
97.9
Duplicates removed (%)
5.5
Number of peaks
860 (qval < 1E-05)

Base call quality data from DBCLS SRA