GSM3734770: 22RV1-DHT-DMSO BRD4; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
BRD4
Cell type
Cell type Class
Prostate
Cell type
22Rv1
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
22RV1 immortalized prostate cancer cell line
chip antibody
BRD4 (Bethyl, Cat A301-985A100, Lot A301-985A50-6)
cell line
22RV1 immortalized prostate cancer cell line
agent
DMSO + 5 nM DHT, 6 hrs
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated, fixed cell pellets and protein-DNA complexes were isolated with antibody. Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation.