GSM3733673: HeLa: PRMT5 ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
Attributes by original data submitter
Sequenced DNA Library
Approximately 1 x 107 cells were used for each ChIP-Seq assay. The chromatin DNA precipitated by polyclonal antibodies against Snail, MTA1, or PRMT5. The DNA was purified with a Qiagen PCR purification kit Libraries were prepared according to Illumina's instructions. The NEBNext Ultra DNA Library Prep Kit for Illumina was used for DNA library construction. After adapter ligation DNA PCR was amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from the agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced following the manufacturer protocols.