GSM3733670: HeLa: input DNA; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
HeLa cell
cell line
HeLa
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Approximately 1 x 107 cells were used for each ChIP-Seq assay. The chromatin DNA precipitated by polyclonal antibodies against Snail, MTA1, or PRMT5. The DNA was purified with a Qiagen PCR purification kit Libraries were prepared according to Illumina's instructions. The NEBNext Ultra DNA Library Prep Kit for Illumina was used for DNA library construction. After adapter ligation DNA PCR was amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from the agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced following the manufacturer protocols.