Curated Sample Data


Genome
ce10
Antigen Class
TFs and others
Antigen
GFP
Cell type Class
Adult
Cell type
Adult

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
Whole organism
strain
isp-1(qm150); ctb-1(qm189); Is[pvha-6::klf-1-yfp]
developmental stage
First day of adulthood
tissue
Whole body
antibody
GFP-TRAP (Chromotek; Cat. #gtma-20; Lot. 81025001MA)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were sonicated and cleared by centrifugation. Protein-DNA complexes were pulled-down using GFP-TRAP (Chromotek). Libraries were prepared by using the Illumina TruSeq DNA Nano Kit with some modifications. The preparation was started with the end repair step of the protocol. Up to 100ng ChIP/Input DNA was used as starting material. After end repair and A-tailing dual indexing adapters were ligated. The products were then purified and amplified (12 PCR cycles for the Input Samples and 15 cycles for the ChIP Samples) to create the final libraries. After validation (Agilent 4200 TapeStation) each single library was quantified by using the Roche KAPA Library Quantification Kit and the Applied Biosystems 7900HT Sequence Detection System. The libraries were then pooled equimolar. Finally the pool was sequenced on one Illumina HiSeq4000 lane 1x51bp (51+8+8).

Platform Information


instrument_model
Illumina HiSeq 4000

External Database Query

Logs in read processing pipeline


Number of total reads
28106398
Reads aligned (%)
82.5
Duplicates removed (%)
41.1
Number of peaks
802 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA