Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ETS1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived cardiac cells
NA
NA

Attributes by original data submitter

Sample

source_name
hESC-derived
tissue
cardiomyocytes
timepoint
d0
chip antibody
ETS1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP for ETS1. Briefly, D0、D2、D5、D9、D14 of hESCs-derived cells were fixed using 1% formaldehyde for 10 min, and 0.125 M glycine was added to stop fixation. Cells were harvested, and DNA was fragmented to 300–500 bp by sonication with a Covaris S220 sonicator. Immunoprecipitation was performed with antibodies conjugated to Dynabeads Protein G beads (1004D, Life Technologies). ChIP DNA was eluted, reverse cross-linked, extracted with phenol/chloroform, and precipitated. For ChIP-Seq, 1 ng ChIP DNA or input DNA was used to generate sequencing libraries using the Nextera XT DNA sample preparation Kit (FC-131-1024,Illumina). Libraries were sequenced on the NextSeq500 sequencer (Illumina) using the 35nt paired-end sequencing protocol.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
17575767
Reads aligned (%)
177.0
Duplicates removed (%)
5.7
Number of peaks
22892 (qval < 1E-05)

hg38

Number of total reads
17575767
Reads aligned (%)
177.7
Duplicates removed (%)
5.7
Number of peaks
22927 (qval < 1E-05)

Base call quality data from DBCLS SRA