Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
DEAF1

Cell type

Cell type Class
Epidermis
Cell type
GMA24A1F
NA
NA

Attributes by original data submitter

Sample

source_name
GMA24A1F
cell type
Keratinocytes cell
chip antibody
anti-Deaf1 (PMID: 14507979)
cell line
GMA24A1F

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Confluent cells were fixed using formaldehyde (1% final concentration) for 10 minutes at room temperature and the EZ-Magma ChIP HiSens kit (EMD Millipore) was used to perform the chromatin inmunoprecipitation library construction was done using NEBNext DNA Ultra II DNA library prep kit for Illumina (New England Biolabs)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
28444441
Reads aligned (%)
1.7
Duplicates removed (%)
40.3
Number of peaks
19 (qval < 1E-05)

hg38

Number of total reads
28444441
Reads aligned (%)
1.8
Duplicates removed (%)
39.4
Number of peaks
10 (qval < 1E-05)

Base call quality data from DBCLS SRA