Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PPP1R10

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

source_name
U2OS cells
cell line
U2OS
chip antibody
PNUTS (Bethyl, A300-439A-T)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq sample preparation was performed as previously described (Herold et al., 2019). Libraries were prepared as previously described (Herold et al., 2019).

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
33676583
Reads aligned (%)
80.8
Duplicates removed (%)
75.1
Number of peaks
3199 (qval < 1E-05)

hg38

Number of total reads
33676583
Reads aligned (%)
81.2
Duplicates removed (%)
74.0
Number of peaks
3287 (qval < 1E-05)

Base call quality data from DBCLS SRA