Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

U2OS cells

cell line

U2OS

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-Seq sample preparation was performed as previously described (Herold et al., 2019). Libraries were prepared as previously described (Herold et al., 2019).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

17531553

Reads aligned (%)

85.3

Duplicates removed (%)

14.9

Number of peaks

751 (qval < 1E-05)

hg19

Number of total reads

17531553

Reads aligned (%)

84.6

Duplicates removed (%)

16.7

Number of peaks

749 (qval < 1E-05)