GSM3722319: ChIP-seq Ishi T47D DMSO Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
ER ChIP-seq
cell line
T-47D
cell type
ER+ breast ductal carcinoma cell line
chip antibody
Santa Cruz ERalpha (HC-20)
treatment
1 hr 0.1% DMSO
genotype
WT
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature. Crosslinking was stopped with 125mM Glycine and cells were washed and scraped in cold PBS. IP was done as described in Reddy et al 2009 using Santa Cruz ERα (HC-20) TruSeq library construction as described in Reddy et al 2009