Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
ER ChIP-seq
cell line
T-47D
cell type
ER+ breast ductal carcinoma cell line
chip antibody
Santa Cruz ERalpha (HC-20)
treatment
1 hr 0.1% DMSO
genotype
WT

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature. Crosslinking was stopped with 125mM Glycine and cells were washed and scraped in cold PBS. IP was done as described in Reddy et al 2009 using Santa Cruz ERα (HC-20) TruSeq library construction as described in Reddy et al 2009

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
47917928
Reads aligned (%)
81.1
Duplicates removed (%)
11.0
Number of peaks
2592 (qval < 1E-05)

hg38

Number of total reads
47917928
Reads aligned (%)
86.0
Duplicates removed (%)
7.1
Number of peaks
3180 (qval < 1E-05)

Base call quality data from DBCLS SRA