GSM3720412: EGR1 A2780 cisplatin; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
EGR1
Cell type
Cell type Class
Gonad
Cell type
A2780
Primary Tissue
Ovary
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
EGR1_A2780_cisplatin
cell line
A2780
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10mins, and followed by glycine for 5 mins. Chromatin DNA was sheared to 200–500 bp average in size by sonication and chromatin was immunoprecipitated with antibodies. Protein G magnetic beads were added and incubated overnight at 4°C. After washing and elution, the protein–DNA complex was reverse-crosslinked by heating at 65°C, and immunoprecipitated DNA was purified by using QIAquick Spin column. ChIP-seq libraries were prepared using the KAPA Library Preparation Kit (KK8201)