Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EGR1

Cell type

Cell type Class
Gonad
Cell type
A2780
Primary Tissue
Ovary
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
EGR1_A2780_vehicle
cell line
A2780

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10mins, and followed by glycine for 5 mins. Chromatin DNA was sheared to 200–500 bp average in size by sonication and chromatin was immunoprecipitated with antibodies. Protein G magnetic beads were added and incubated overnight at 4°C. After washing and elution, the protein–DNA complex was reverse-crosslinked by heating at 65°C, and immunoprecipitated DNA was purified by using QIAquick Spin column. ChIP-seq libraries were prepared using the KAPA Library Preparation Kit (KK8201)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
17948432
Reads aligned (%)
49.7
Duplicates removed (%)
2.2
Number of peaks
1215 (qval < 1E-05)

hg38

Number of total reads
17948432
Reads aligned (%)
50.6
Duplicates removed (%)
1.3
Number of peaks
1176 (qval < 1E-05)

Base call quality data from DBCLS SRA