GSM3718072: D425J1 resistant H3K4me3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Neural
Cell type
D425
NA
NA
Attributes by original data submitter
Sample
source_name
D425
cell type
D425 tolerant
treatment
JQ1
ip antibody
H3K4me3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sensitive and drug-tolerant D425 and D458 were passaged in DMSO or JQ1 (1 µM) for 24 hours. Sheared chromatin from each cell line was subjected to ChIP-sequencing as previously described62 , enriching for H3K27ac (Cell Signaling Technologies, 8173S)), H3K4me3 (Cell Signaling Technologies, 9751S), Histone H3 (Cell Signaling 4499S) and BRD4 (Bethyl, A301-985A100). ChIP libraries were indexed, pooled and sequenced on Illumina Hi-seq-2000 sequencers.