Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
pho

Cell type

Cell type Class
Embryo
Cell type
Mesoderm
NA
NA

Attributes by original data submitter

Sample

source_name
mesoderm stage 14-17
genotype/variation
twi-INTACT, Mef2>Nslmb,GFP-Ubx
developmental stage
mesoderm stage 14-17
cell type
mesoderm
chip antibody
Rb-Pho 2-382 (gift from Jürg Müller)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
tissue specific nuclei were extracted vis the INTACT method (Steiner et al., 2012) Samples were amplified using the ThruPLEX DNA-Seq Kit (Rubicon). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
33781979
Reads aligned (%)
90.2
Duplicates removed (%)
46.6
Number of peaks
2496 (qval < 1E-05)

dm6

Number of total reads
33781979
Reads aligned (%)
89.3
Duplicates removed (%)
49.3
Number of peaks
3552 (qval < 1E-05)

Base call quality data from DBCLS SRA