Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
HPC-7
NA
NA

Attributes by original data submitter

Sample

source_name
shStag2 inducible shRNA in HPC
cell line
HPC-7
cell type
Hematopoietic stem and progenitors
genotype
HPC_shStag2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
x-linking was performed with 1% Formaldehyde. Lysates were clarified from sonicated nuclei and chromatin proteins were isolated with antibodies. ChIP-Seq libraries were prepared for sequencing using a TruSeq ChIP Sample Prep Kit (Illumina) and standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
62226360
Reads aligned (%)
97.1
Duplicates removed (%)
57.2
Number of peaks
13136 (qval < 1E-05)

mm9

Number of total reads
62226360
Reads aligned (%)
96.8
Duplicates removed (%)
57.2
Number of peaks
13892 (qval < 1E-05)

Base call quality data from DBCLS SRA