Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Embryo
Cell type
Embryonic heart
NA
NA

Attributes by original data submitter

Sample

source_name
NKX2-5_E9.25_heart
strain
C57BL/6
developmental stage
E9.25 heart
chip antibody
H3K4me3, Millipore, 04-745, Lot: 3068440

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The hearts of E9.25 embryo was isolated and directly used for in situ ChIP After PCR enrichment using 2X KAPA master mix, products were purified and selected by AMPure XP beads for 200-1000 bp.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

mm10

Number of total reads
13753359
Reads aligned (%)
30.7
Duplicates removed (%)
40.7
Number of peaks
2962 (qval < 1E-05)

mm9

Number of total reads
13753359
Reads aligned (%)
30.7
Duplicates removed (%)
40.7
Number of peaks
2947 (qval < 1E-05)

Base call quality data from DBCLS SRA