Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
E6.5 embryos
NA
NA

Attributes by original data submitter

Sample

source_name
IgG_E6.5_embryo
strain
C57BL/6
developmental stage
E6.5_embryo
chip antibody
IgG, AB clonal, WH086883, AC005

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
E6.5, E7.0 and E7.75 embryo were directly isolated form uterus and used for in-situ ChIP After PCR enrichment using 2X KAPA master mix, products were purified and selected by AMPure XP beads for 200-1000 bp.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

mm10

Number of total reads
10485739
Reads aligned (%)
47.4
Duplicates removed (%)
38.7
Number of peaks
66 (qval < 1E-05)

mm9

Number of total reads
10485739
Reads aligned (%)
47.4
Duplicates removed (%)
38.6
Number of peaks
61 (qval < 1E-05)

Base call quality data from DBCLS SRA