Curated Sample Data


Genome
mm9
Antigen Class
Histone
Antigen
H3K27ac
Cell type Class
Embryo
Cell type
E7.0 embryos

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
H3K27ac_E7.0_embryo
strain
C57BL/6
developmental stage
E7.0_embryo
chip antibody
H3K27ac, Abcam, ab4729, Lot: GR3205526-1

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
E6.5, E7.0 and E7.75 embryo were directly isolated form uterus and used for in-situ ChIP After PCR enrichment using 2X KAPA master mix, products were purified and selected by AMPure XP beads for 200-1000 bp.

Platform Information


instrument_model
Illumina NovaSeq 6000

External Database Query

Logs in read processing pipeline


Number of total reads
7705065
Reads aligned (%)
76.2
Duplicates removed (%)
20.5
Number of peaks
4238 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA