Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Embryo
Cell type
E7.0 embryos
NA
NA

Attributes by original data submitter

Sample

source_name
H3K4me3_E7.0_embryo
strain
C57BL/6
developmental stage
E7.0_embryo
chip antibody
H3K4me3, Millipore, 04-745, Lot: 3068440

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
E6.5, E7.0 and E7.75 embryo were directly isolated form uterus and used for in-situ ChIP After PCR enrichment using 2X KAPA master mix, products were purified and selected by AMPure XP beads for 200-1000 bp.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

mm10

Number of total reads
18367132
Reads aligned (%)
66.4
Duplicates removed (%)
70.1
Number of peaks
14842 (qval < 1E-05)

mm9

Number of total reads
18367132
Reads aligned (%)
66.4
Duplicates removed (%)
70.1
Number of peaks
14834 (qval < 1E-05)

Base call quality data from DBCLS SRA