SRX5637693
GSM3711189: H3K4me3 E6.5 embryo rep1; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Embryo
Cell type
E6.5 embryos
NA
NA

Attributes by original data submitter

Sample

source_name
H3K4me3_E6.5_embryo
strain
C57BL/6
developmental stage
E6.5_embryo
chip antibody
H3K4me3, Millipore, 04-745, Lot: 2872328

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
E6.5, E7.0 and E7.75 embryo were directly isolated form uterus and used for in-situ ChIP After PCR enrichment using 2X KAPA master mix, products were purified and selected by AMPure XP beads for 200-1000 bp.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
6779079
Reads aligned (%)
37.7
Duplicates removed (%)
42.0
Number of peaks
5711 (qval < 1E-05)

mm9

Number of total reads
6779079
Reads aligned (%)
37.6
Duplicates removed (%)
42.0
Number of peaks
5697 (qval < 1E-05)

Base call quality data from DBCLS SRA