Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RUNX1

Cell type

Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease

Attributes by original data submitter

Sample

source_name
Engineered MCF10A cells (ATCC® CRL-10317)
cell line
MCF10A
genotype
RUNX1-deleted MCF10A cell line
treatment
transduced RUNX1-KO MCF10A cells with lentiviruses that inducibly express RUNX1; treated RUNX1-inducible MCF10A cells with doxycycline
chip antibody
anti-RUNX1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP DNA was extracted by standard phenol approach. TruSeq ChIP Library Prep Kit (IP-202-1012)

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
91726821
Reads aligned (%)
84.9
Duplicates removed (%)
12.1
Number of peaks
7206 (qval < 1E-05)

hg38

Number of total reads
91726821
Reads aligned (%)
87.4
Duplicates removed (%)
10.4
Number of peaks
7924 (qval < 1E-05)

Base call quality data from DBCLS SRA