Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FLI1

Cell type

Cell type Class
Bone
Cell type
A-673
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
Rhabdomyosarcoma

Attributes by original data submitter

Sample

source_name
A673/TR/shEF Ewing sarcoma cells
chip-antibody
FLI1 rabbit polyclonal antibody (abcam, ab15289-ChIP grade)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA-protein cross-linking was performed in the presence of 1% of paraformaldehyde. Cell lysis, chromatin shearing, immunoprecipitation and DNA purification was performed with reagents from iDealChIP-seq kit for Transcription Factors ChIP-seq - HiSeq2500 (Illumina) using 100 bp single-end sequencing.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
46745500
Reads aligned (%)
94.2
Duplicates removed (%)
50.7
Number of peaks
10659 (qval < 1E-05)

hg38

Number of total reads
46745500
Reads aligned (%)
95.2
Duplicates removed (%)
49.2
Number of peaks
10992 (qval < 1E-05)

Base call quality data from DBCLS SRA