Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Bone

Cell type

A-673

Primary Tissue

Skeletal Muscle

Tissue Diagnosis

Rhabdomyosarcoma

Sample

source_name

A673/TR/shEF Ewing sarcoma cells

chip-antibody

input DNA

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA-protein cross-linking was performed in the presence of 1% of paraformaldehyde. Cell lysis, chromatin shearing, immunoprecipitation and DNA purification was performed with reagents from iDealChIP-seq kit for Transcription Factors ChIP-seq - HiSeq2500 (Illumina) using 100 bp single-end sequencing.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

45596183

Reads aligned (%)

97.7

Duplicates removed (%)

3.5

Number of peaks

1606 (qval < 1E-05)

hg19

Number of total reads

45596183

Reads aligned (%)

96.5

Duplicates removed (%)

4.9

Number of peaks

941 (qval < 1E-05)