GSM3691939: MSC input Rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Others
Cell type
Mesenchymal stem cells
NA
NA
Attributes by original data submitter
Sample
source_name
MSC_input
msc donor
017508A
cell type
human mesenchymal stem cells
chip antibody
none
differentiation time point
0
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from hMSCs and differentiated chondrocytes were extracted using the Diagenode iDeal histone ChIP-seq kit (Diagenode SA, Ougrée, Belgium). Extracted chromatin was sonicated using a Diagenode Bioruptor Standard or Bioruptor Pico to an average size of 200-500bp, using 15 sonication cycles (30s on/30s off). Replicate 1: Diagenode MicroPLEX v2 kit, replicate 2: NEBNext Ultra II kit (New England Biolabs, Hitchin, UK)