Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Breast

Cell type

HCC2157

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

breast cancer cells

cell line

HCC2157

cell type

breast cancer cells

chip antibody

H3K4me3 (Abcam, ab1012)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

To isolate chromatin, formaldehyde-cross-linked nuclei were sonicated using a Covaris focused-ultrasonicator. Following ChIP performed with specific antibodies and Dynabeads® Protein G (Invitrogen) ChIP DNA was subjected to end repair, A-tailing, ligation of Illumia single end adaptor. Following PCR amplification for 18 cycles, the final library underwent gel purification.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

25453428

Reads aligned (%)

98.7

Duplicates removed (%)

36.8

Number of peaks

23441 (qval < 1E-05)

hg19

Number of total reads

25453428

Reads aligned (%)

97.8

Duplicates removed (%)

38.5

Number of peaks

23417 (qval < 1E-05)