GSM3687833: ChIP-Seq A549 p-Babe LKB1 rep3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CRTC2
Cell type
Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
A549 cells
cell type
NSCLC cells
transduction
retrovirus mediated transduction of pBabe-LKB1 (WT)
expression
stable expression of pBabe-LKB1(WT) vector selected with puromycin
chip-antibody
6865 anti-CRTC2 rabbit serum
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody as indicated ChIP-seq libraries were constructed with NEBNext ChIPseq library kit (NEB) according to manufacturer's instructions.