Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CRTC2

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549 cells
cell type
NSCLC cells
transduction
retrovirus mediated transduction of pBabe-LKB1 (WT)
expression
stable expression of pBabe-LKB1(WT) vector selected with puromycin
chip-antibody
6865 anti-CRTC2 rabbit serum

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody as indicated ChIP-seq libraries were constructed with NEBNext ChIPseq library kit (NEB) according to manufacturer's instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
27668655
Reads aligned (%)
86.3
Duplicates removed (%)
8.9
Number of peaks
22846 (qval < 1E-05)

hg38

Number of total reads
27668655
Reads aligned (%)
87.1
Duplicates removed (%)
8.5
Number of peaks
22840 (qval < 1E-05)

Base call quality data from DBCLS SRA