Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SPI1

Cell type

Cell type Class
Blood
Cell type
Monocytes
MeSH Description
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Attributes by original data submitter

Sample

source_name
MO4h_PU.1-ChIP
donr id
donorO
tissue/cell type
peripheral blood
cell subtype
primary monocytes
chip antibody
PU.1 (Santa Cruz, #sc-352X)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPseq was essentially done as described (Pham et al. 2012). Libraries were generated as described (Pham et al. 2012)

Sequencing Platform

instrument_model
Illumina HiSeq 3000

hg38

Number of total reads
25816054
Reads aligned (%)
96.7
Duplicates removed (%)
39.8
Number of peaks
10671 (qval < 1E-05)

hg19

Number of total reads
25816054
Reads aligned (%)
95.7
Duplicates removed (%)
41.3
Number of peaks
10707 (qval < 1E-05)

Base call quality data from DBCLS SRA