Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Mast Cells

MeSH Description

Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.

Sample

source_name

BDMC_Input-ChIP

donr id

donorH

tissue/cell type

breast tissue

cell subtype

breast-derived primary mast cells

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIPseq was essentially done as described (Pham et al. 2012). Libraries were generated as described (Pham et al. 2012)

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

36020710

Reads aligned (%)

98.8

Duplicates removed (%)

37.6

Number of peaks

1012 (qval < 1E-05)

hg19

Number of total reads

36020710

Reads aligned (%)

98.0

Duplicates removed (%)

39.1

Number of peaks

1232 (qval < 1E-05)