Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
Yolk Sac
MeSH Description
The first of four extra-embryonic membranes to form during EMBRYOGENESIS. In REPTILES and BIRDS, it arises from endoderm and mesoderm to incorporate the EGG YOLK into the DIGESTIVE TRACT for nourishing the embryo. In placental MAMMALS, its nutritional function is vestigial; however, it is the source of INTESTINAL MUCOSA; BLOOD CELLS; and GERM CELLS. It is sometimes called the vitelline sac, which should not be confused with the VITELLINE MEMBRANE of the egg.

Attributes by original data submitter

Sample

source_name
Visceral yolk sac endoderm
tissue
Visceral yolk sac endoderm
age
E12.5
brackground strain
CAST/EiJxFVB/NJ
strain
RSDEL
genotype
++
Sex
pooled male and female
chip antibody
H3K27me3 Jenuwein antibody 6523, 5th bleed

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was extracted from TRIzol using standard protocols. The Illumina TruSeq kit was used to create polyA+ libraries from total RNA. We generated strand-specific libraries for tissues collected form the F1 crosses. All the libraries were run on a Bioanalyzer to assess fragment size, and concentration and sequenced on a HiSeq 2000

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
22205809
Reads aligned (%)
91.3
Duplicates removed (%)
31.1
Number of peaks
111 (qval < 1E-05)

mm9

Number of total reads
22205809
Reads aligned (%)
91.2
Duplicates removed (%)
31.2
Number of peaks
180 (qval < 1E-05)

Base call quality data from DBCLS SRA