Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me2

Cell type

Cell type Class
Blood
Cell type
Multiple Myeloma
MeSH Description
A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.

Attributes by original data submitter

Sample

source_name
Multiple myeloma
cell type
Multiple myeloma
phenotype
High levels of MMSET
passage
15-20
chip antibody
Millipore 07-369

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) was performed according to the manufacture's protocol (ChIP kit, Millipore) with the following modifications: 107 cells were used per sample. After cell lysis, extracts were subjected to sonication with a 60 Sonic Dismembrator (Fisher Scientific) at 4°C for 20 cycles, cycling ON for 20 seconds and OFF for 1 minute at 4.5 power leading to a DNA average size of 200 bp. Immunoprecipitations were performed overnight at 4°C using 5 μg of antibody. Imunoprecipitated chromatin was extracted using QIAquick PCR Purification Kit (QIAGEN). 10 ng of input and ChIP material was processed using the Illumina kit (IP-102-1001)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
239167930
Reads aligned (%)
98.8
Duplicates removed (%)
7.9
Number of peaks
3183 (qval < 1E-05)

hg19

Number of total reads
239167930
Reads aligned (%)
98.1
Duplicates removed (%)
9.4
Number of peaks
2227 (qval < 1E-05)

Base call quality data from DBCLS SRA