Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Neural

Cell type

Anterior temporal cortex

NA

NA

Sample

source_name

Fetal anterior temporal cortex

developmental stage

Adult

tissue

brain (adult anterior temporal cortex)

chip antibody

CTCF (Abcam, ab70303)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, nuclei were extracted and chromatins were fragmented by sonication. The TF/histone-DNA complexes were isolated by antibody. All ChIP-seq libraries were generated following a ChIPmentation protocol with Nextera adapters.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

41569341

Reads aligned (%)

99.3

Duplicates removed (%)

57.2

Number of peaks

5049 (qval < 1E-05)

hg19

Number of total reads

41569341

Reads aligned (%)

99.0

Duplicates removed (%)

57.9

Number of peaks

5030 (qval < 1E-05)